The role of staining techniques in seminological analysis of mammalian semen

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Title The role of staining techniques in seminological analysis of mammalian semen
Tytuł równoległy: Rola technik barwienia w ocenie seminologicznej nasienia ssaków
Autor: Banaszewska, Dorota; Andraszek, Katarzyna; Zdrowowicz, Ewa; Czubaszek, Magdalena; Walczak-Jędrzejowska, Renata
URI: http://hdl.handle.net/11331/400
Date: 2015-12-11
Źródło: Folia Pomeranae Universitatis Technologiae Stetinensis. Seria: Agricultura, Alimentaria, Piscaria et Zootechnica. - 2015, 320 (35/3), s. 5-20
Abstract: The most important semen parameters are the concentration, motility and morphology of sperm cells. Sperm morphology is regarded as the most reliable parameter for predicting fertility in males. A problem in evaluating sperm morphology and morphometry is the lack of standardization of staining techniques. The staining procedure and reagents used can significantly affect the morphometric parameters of the sperm cell. The use of stains with different pH or osmotic pressure, as well as the duration of the procedure, may influence the shape and size of the sperm, and thus the result of the morphological evaluation of the semen. It is necessary to develop an evaluation procedure for sperm morphology and morphometry that will minimize the changes in the structure of the evaluated semen in relation to the native semen.

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