Age-dependent change in the morphology of nucleoli and methylation of genes of the nucleolar organizer region in Japanese Quail (Coturnix japonica) model (Temminck and Schlegel, 1849) (Galliformes: Aves)

Repozytorium Centrum Otwartej Nauki

Pokaż pełny rekord

Tytuł Age-dependent change in the morphology of nucleoli and methylation of genes of the nucleolar organizer region in Japanese Quail (Coturnix japonica) model (Temminck and Schlegel, 1849) (Galliformes: Aves)
Autor: Andraszek, Katarzyna; Gryzińska, Magdalena; Wójcik, Ewa; Knaga, Sebastian; Smalec, Elżbieta
URI: http://hdl.handle.net/11331/371
Data: 2014
Źródło: Folia Biologica. - Vol. 62, nr 4 (2014), s. 293-300
Streszczenie: Nucleoli are the product of the activity of nucleolar organizer regions (NOR) in certain chromosomes. Their main functions are the formation of ribosomal subunits from ribosomal protein molecules and the transcription of genes encoding rRNA. The aim of the study was to determine the shape of nucleoli and analyse methylation in the gene RN28S in the spermatocytes of male Japanese quail (Coturnix japonica) in two age groups. Nucleoli were analysed in cells of the first meiotic prophase. Their number and shape were determined and they were classified as regular, irregular or defragmented. In the cells of the young birds no defragmented nucleoli were observed, with regular and irregular nucleoli accounting for 97% and 3%, respectively. In the cells of older birds no regular nucleoli were observed, while irregular and defragmented nucleoli accounted for 37% and 67%, respectively. MSP (methylation-specific PCR) showed that the gene RN28S is methylated in both 15-week-old and 52-week-old quails. In recent years an association has been established between nucleolus morphology and cellular ageing processes.

Pliki tej pozycji

Pozycja umieszczona jest w następujących kolekcjach

Szukaj w repozytorium


Szukanie zaawansowane

Przeglądaj

Moje konto

Statystyki