Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)

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Title Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)
Autor: Kot, Barbara; Szweda, Piotr; Lada, K.; Rytel, E.; Gucwa, Katarzyna Magdalena; Piechota, Małgorzata; Wolska, Marzena Katarzyna
URI: http://hdl.handle.net/11331/3567
https://doi.org/10.2478/pjvs-2014-0044
Date: 2014
Źródło: Polish Journal of Veterinary Sciences. Vol. 17, No. 2 (2014), p.321 -329
Abstract: The molecular-typing strategy, ERIC-PCR was used in an attempt to determine the genomicrelationship of 28P.aeruginosastrains isolated from faeces of healthy bovine, bovinemastitisandfrom faeces of hospital patients as well as from environment. ERIC-PCR fingerprinting revealedlarge molecular differentiation within this group of isolates. Twenty two out of 28 strains testedgenerated unique patterns of DNA bands and only three genotypes consisted of two isolates eachwere identified.We also tested theP.aeruginosaisolates for their ability to form a biofilm on abiotic surfacesincluding polyvinylchloride and polystyrene. Different biofilm-forming abilities were demonstratedamong strains; however, most of them (64.3%) showed moderate-biofilm forming ability. The strainswith increased swimming and twitching motility displayed elevated biofilm formation. However,a negative correlation was found between slime and initial biofilm production. On the basis of theresults obtained, we suggest that there are no major differences in phenotypic properties betweenP.aeruginosastrains isolated from different sources.

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Uznanie autorstwa 3.0 Polska Except where otherwise noted, this item's license is described as Uznanie autorstwa 3.0 Polska

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