Coexistence of Borrelia burgdorferi s. l. genospecies within Ixodes ricinus ticks from central and eastern Poland

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Title Coexistence of Borrelia burgdorferi s. l. genospecies within Ixodes ricinus ticks from central and eastern Poland
Autor: Sytykiewicz, Hubert; Karbowiak, Grzegorz; Chorostowska-Wynimko, Joanna; Szpechciński, Adam; Supergan-Marwicz, Marta; Horbowicz, Marcin; Szwed, Magdalena; Czerniewicz, Paweł; Sprawka, Iwona
Date: 2015
Źródło: Acta Parasitologica. Vol. 60, no. 4 (2015), p. 654-661
Abstract: The purpose of the study was to assess the prevalence and coinfection rates of Borrelia burgdorferisensu lato genotypes inIxodes ricinus(L.) ticks sampled from diverse localities in central and eastern regions of Poland. In years 2009-2011, questingnymphs and adults ofI. ricinuswere collected using a flagging method at 18 localities representing distinct ecosystem types:urban green areas, suburban forests and rural woodlands. Molecular detection of B. burgdorferis.l. genospecies was based onamplification of a flagene using nested PCR technique, subsequent PCR-RFLP analysis and bidirectional sequencing. It wasrevealed that 45 samples (2.1%) harboured two different B. burgdorferis.l. genospecies, whereas triple infections with variousspirochetes was found in 11 (0.5%) individuals. Generally, the highest average coinfection rates were evidenced in arachnidsgathered at rural woodlands, intermediate at suburban forests, while the lowest were recorded at urban green areas. Overall, sin-gle spirochete infections were noted in 16.3% (n = 352/2,153) ticks. Importantly, it is the first report evidencing the occurrenceof Borrelia miyamotoi(0.3%, n = 7/2153) in I. ricinuspopulations within central Poland. Circumstantial variability of B. burgdorferis.l. genospecies in the common tick individuals sampled at various habitat types in central and eastern Polandwas displayed. The coexistence of two or three different spirochete genospecies in single adult ticks, as well as the presence ofB. miyamotoiwere demonstrated. Therefore, further studies uncovering the co-circulation of the tested bacteria and other humanpathogens in I. ricinusticks are required

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